Tsingke tse005 2×t5 super pcr mix colony

WebJul 18, 2024 · Polymerase Chain Reaction (PCR) was performed in 12 μl reaction volumes containing 2 template DNA, 5 μl of 2 × T5 Super PCR Mix (PAGE; TSINGKE, Beijing, China), 1 μl of 10 μmol/μL primer pairs, and 4 μl of double distilled H 2 O. Target DNA segments were amplified with the following program, 95°C for 5 min, followed by 35 cycles of 95°C for 10 … Web2 O PCR primers OneTaq Master Mix 25 µl PCR Primer 200 nM H 2 O to 50 µl Note: If OneTaq Hot Start Quick-Load 2X Master Mix is used, reactions can be set up at room temperature. If OneTaq Quick-Load 2X Master Mix is used, reactions should be set up on ice. INITIAL DENATURATION PCR Primer 2 minutes 30 CYCLES 94°C 15–30 seconds …

Identification and Characterization of the ERF Subfamily

WebMay 25, 2024 · We use a homemade 2× PCR master mix containing DNA polymerase, buffer, Mg 2+, dNTPs, and DMSO (see Subheading 2) to which are added PCR primers to a final concentration of 1 μM and water. We prepare 110% of the theoretical required volume, which is calculated as the total volume of each PCR reaction times the number of clones, … http://www.transgenbiotech.com/data/upload/image/20241231/1609386732127122.pdf i play on a toaster lyrics https://guineenouvelles.com

TOROGreen® Yeast colony PCR Kit - toroivd.com

WebMar 5, 2024 · Then, colony PCR was used to identify the bacterial strain. Briefly, about 2 μL of bacteria were picked into a 50 μL 2 × T5 Super PCR Mix (Colony) system (TSINGKE Biological Technology, Beijing, China) and then amplified and sequenced using primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTA CGACTT-3′). WebTSINGKE TSE005 2×T5 Super PCR Mix (Colony) 销售价: ￥395.00. 市场价: ￥395.00. 编号: 1216162706837. 货号: TSE005. Web2×T5 Super PCR Mix (Colony) TSE005 产品说明： 目录号： T5 DNA 聚合酶是新型DNA 聚合酶，由经过改造的Taq DNA 聚合酶融合Sso7d DNA结合域而得来，具有极高的扩增 速度 … i play on the computer

Platinum™ SuperFi II PCR Master Mix - Thermo Fisher Scientific

Category:PCR Product Purification and Sanger Sequencing Submission

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Tsingke tse005 2×t5 super pcr mix colony

PCR Product Purification and Sanger Sequencing Submission

Web2×T5 Super PCR Mix（Colony）. ¥ 395.00. 货号：TSE005. 规格：. 5×1 mL. 前往科邦邦商城下单. 产品简介. 合成基因组产品及服务. 引物合成. WebPublish time 2024-09-12 17:24. TOROGreen® Yeast colony PCR Kit is used to amplify fragments from the yeast genome as well as transformed plasmids. The same as E …

Tsingke tse005 2×t5 super pcr mix colony

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WebPick one colony and dissolve in 100 μL media 2 . Take 1 μL as DNA sample for PCR 3 . ... Dream Taq PCR Master Mix(2X) 12.5 Forward Primer 0.1-1.uM Reverse primer 0.1-1.uM … WebCitations & References. PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Simply add template and primers, …

http://tsingke-shop.oss-cn-beijing.aliyuncs.com/TSE008-2%C3%97T5%20Super%20PCR%20Mix%28Basic%29%E8%AF%B4%E6%98%8E%E4%B9%A6V1.1.3.pdf Web丁香通为您找到1条pcr非特异扩增产物多大信息，包括pcr非特异扩增产物多大报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购pcr非特异扩增产物多大,上丁香通移动站。

Web2) Pick 5 transformant colonies. 3) Grow each colony in respective antibiotic containing LB broth for 5-6 hours. 4) Took 500ul of culture and centrifuge. 5) Resuspend in 50ul of nuclease free ... Web2 ×T5 Super PCR Mix (Colony) 5. Initial denaturation. 98. 30s. 1 × . Forward primer . 0.2. Denaturation. 98. 10s. 29 × . Feverse primer . 0.2. Annealing. T m-5. 30s. Template. 2. …

Web2 O PCR primers OneTaq Master Mix 25 µl PCR Primer 200 nM H 2 O to 50 µl Note: If OneTaq Hot Start Quick-Load 2X Master Mix is used, reactions can be set up at room …

WebThe SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H 2 O. • TransTaq ® High Fidelity (HiFi) PCR SuperMix offers 18 … i play on the computer in spanishWebSep 8, 2015 · PrimeSTAR Max polymerase (2× Master Mix, Takara Bio) was used to generate both altered plasmids. PCR reactions were digested with DpnI for ~2 hours at 37°C and purified with Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). 1 μl of the purified product (125–150 ng DNA) was combined with 25 μl NEB 5-alpha chemically competent … i play outside in frenchWebUse of 2 Step PCR is recommended when the primer's Tm values are >68°C Mg2+ The final concentration of Mg 2+ in 1X I-5™ PCR Master Mix is 2 mM. Add additional Mg as … i play pokemon go everyday piano sheet musicWebcombined with 100 µL of Binding Buffer). Mix thoroughly. Note. If PCR mixture contains primer-dimers, purification without isopropanol is recommended. However, the yield of … i play perthWebtsingke-shop.oss-cn-beijing.aliyuncs.com i play outside pahttp://tsingke-shop.oss-cn-beijing.aliyuncs.com/TSE005-2%C3%97T5%20Super%20PCR%20Mix%20%28Colony%29%E8%AF%B4%E6%98%8E%E4%B9%A6V1.1.2.pdf i play pinochle with a horseWebJan 6, 2024 · Then, qRT-PCR was carried out on the CFX96 instrument (Bio-Rad, USA) with Ssofast EvaGreen Supermix Kit in a 20 µl reaction volume containing 0.8 µl of each primer, … i play pokemon go lyric