Thp1 transwell
WebMay 1, 2015 · THP-1 cells are grown in suspension in RPMI/FCS media, and activation is performed once plated into transwell inserts. Inserts are handled at the sterile edges of … WebFeb 8, 2006 · A higher number of RA FLS co-cultured with differentiated THP-1 cells (1055.67 ± 63.06 cells/filter) were found to have invaded through transwell chambers than that of RA FLS co-cultured with undifferentiated THP-1 cells (726 ± 83.86 cells/filter) (P < 0.05; Figure 4b,d).
Thp1 transwell
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WebToll-like receptors (TLRs) induce the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and autophagy through the TNF (Tumor necrosis factor) … WebDec 31, 2024 · Lipopolysaccharydes (LPS) are responsible for the intestinal inflammatory reaction, as they may disrupt tight junctions and induce cytokines (CKs) secretion. …
WebAug 8, 2015 · In the co-culture experiments, THP-1 monocytes were differentiated in 6 Transwell inserts (membrane pore size of 0.4 μm, Corning, #3450). Macrophages and … WebApr 7, 2024 · RAGE knockdown inhibited chemotaxis and adhesion of THP-1 cells exposed to intermittent hypoxia. Knockdown of RAGE in THP-1 cells was verified via qRT-PCR (a) …
WebNov 17, 2024 · Briefly, 2.5 × 10 4 MDA-MB-231 or Hs578T cells pre-treated with CXCL7 or pre-co-cultured with THP-1 or U937 cells were suspended in DMEM (300 μL) and seeded … WebA Transwell migration assay was employed to evaluate cell invasion. 24-well Transwell plates with 8-μm-diameter filters (Coring, NY, USA) were utilized. Approximately 2×105 …
WebSep 4, 2024 · A co-culture system of THP-1 cells and SW480 cells was established using 24-well transwell plates (Millipore). SW480 cells in the logarithmic growth phase were …
Web1.一磷酸脱氧 鸟苷在制备提高放射治疗对结直肠癌的杀伤力的药物中的应用。 2.根据权利要求1所述应用，其特征在于，所述药物与放射治疗联合提高结直肠癌杀伤力。 3.根据权利要求2所述应用，其特征在于，所述提高肿瘤杀伤力通过提高肿瘤组织中巨噬细胞数量和提高巨噬细胞向结直肠癌细胞的 ... gravely leaf loaderWebthe migration of another THP1 monocyte cell line that stably expresses GFP for visualization.41 The top chambers of the transwells were filled with equal numbers of a … chn to bdtWebsignal-to-ratio for THP-1 chemotaxis was achieved at approximately 25 minutes following exposure to the chemoattractant in the bottom chamber. Further incubation led to a … gravely lawn tractors dealersWebDec 1, 2024 · To initiate the co-culture, the transwell filters with Caco-2 cells were transferred onto well plates containing 24 h-differentiated THP-1 cells without additional … gravely lawn tractor with wing mowerWebWestern blot was performed to detect JMJD1A, JMJD1C, H3K9me2, and H3K9me3 expressions. A transwell migration assay was conducted to detect THP1-Mφ chemotaxis toward chemokine ligand 3 (CCL3). A chromatin immunoprecipitation (ChIP) assay was performed to detect H3K9me2-CCR5 complexes in THP1- Mφs. gravely lawn tractorsWebMay 22, 2024 · In brief, THP-1-derived macrophages (1 × 10 5 cells/well) or Cal-27 cells (1 × 10 5 cells/well) were placed into the upper chamber of transwell inserts with 100 μL of serum-free medium. gravely leaf blowerWebMar 30, 2024 · Last, transwell migration assays showed that IL-1β-primed SKOV3-TCM significantly increased the recruitment of THP1/PBMC Mφ, compared to control TCM and … gravely leaf blower attachment