Flag-tag mouse monoclonal antibody

WebDetection was performed with ANTI-FLAG® M2 monoclonal primary antibody, anti-mouse-HRP secondary antibody, and ECL™ chemiluminescent substrate. Figure 3. Comparative Sensitivity of 3xFLAG®. Each protein fusion tag was cloned separately on the C terminal of GST. The purified proteins were diluted from 1 ug to 0.01 ng and analyzed. WebMouse Antibody Monoclonal. Introduction. A fusion tag called FLAG consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. …

DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to …

WebJan 21, 2024 · For the primary antibody reaction, DYKDDDDK Tag (D6W5B) Rabbit monoclonal antibody (1:1000, 14793; Cell Signal Technology, Beverly, MA, USA) and anti-C23 (NCL) mouse monoclonal (1:500, sc-55486; Santa Cruz Biotechnology, Dallas, TX, USA) were used in the antibody solution, and the mixture was allowed to react with the … WebThe antibody recognizes the DYKDDDDK peptide, which is the same epitope recognized by Sigma's Anti-FLAG ® antibodies, fused to either the amino-terminus or carboxy-terminus of the target protein. Species … no rear heat in 2010 nissan armada https://guineenouvelles.com

Flag Tag Monoclonal Antibody - Cusabio

WebThis antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. Protocols Standard Protocols Click here to view our Standard Protocols Publications All (610) WB … WebKey features and details. Mouse monoclonal [FG4R] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: WB, ELISA, Dot blot, IP, In situ hybridization. Reacts with: … WebDYKDDDDK Tag Monoclonal Antibody ... -Mouse Monoclonal Antibody Acquista DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin, Invitrogen™ su. Italiano English; Change Country. Ricevi tutte le ultime informazioni Centro di Assistenza e Supporto › Italiano English; Change Country; 0. No offer available; Struttura chimica ... no rear windshield cvc

Purified anti-DYKDDDDK Tag Antibody anti-DYKDDDDK - L5

Category:FLAG M2 monoclonal antibody (DYKDDDDK tag) produced in mouse…

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Flag-tag mouse monoclonal antibody

FLAG M2 mouse monoclonal antibody (DYKDDDDK tag).

WebMouse monoclonal [F-tag-01] antibody to DDDDK Tag. Applications: ICC, WB: Reactivity: Species independent: Conjugate: Unconjugated: ... Anti-FLAG Tag Antibody [FG4R] (A85282) 10µg - 200µg / £90 – £280 1 Citation. Description: Mouse monoclonal (FG4R) antibody to DYKDDDDK Tag. Applications: http://www.abpbiosciences.com/product/flag-tag/

Flag-tag mouse monoclonal antibody

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WebThe ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody …

WebFLAG Tag Rabbit Polyclonal Antibody: 100μl: AF5051: Flag Tag Mouse Monoclonal Antibody: 50μl: AF519: Flag抗体(小鼠单抗) >40次: AF0033: Myc Tag Mouse Monoclonal Antibody: 100μl: AF5054: Myc Tag Mouse Monoclonal Antibody: 50μl: AF6513: c-Myc Rabbit Polyclonal Antibody: 50μl: AM926: WebA FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence.

WebKey features and details. PerCP Mouse monoclonal [M2] to DDDDK tag (Binds to FLAG® tag sequence) Suitable for: Flow Cyt. Reacts with: Species independent. Conjugation: PerCP. Ex: 482nm, Em: 675nm. Isotype: IgG1. WebThe Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides. Packaging 0.2, 1, 1 mg Application

WebSpecificity: Flag-Tag Mouse Monoclonal antibody detects endogenous levels of C-terminal, internal, and N-terminal Flag-tagged proteins. Immunogen: A synthesized …

WebWestern blot was performed using HRP-conjugated Anti-DYKDDDDK Mouse Monoclonal Antibody (Product # MA1-91878-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 50 µg (Lane 1), empty vector control, 50 µg (Lane 2), HA-H3-FLAG, 40 µg (Lane 3), HA-H3-FLAG, 20 µg (Lane 4), HA … no rear fender on motorcycleWebConfocal image showing positive staining for FLAG® on HEK-293T cells transfected with DDDDK-tagged PFKFB3 expression vector. Mouse … how to remove green hair dye from skinWebThe MA1-142 antibody (clone L5) has been successfully used as a detection antibody in a direct ELISA with FLAG-tagged protein-coated plates. Similar Epitope as the FLAG tag … no really songWebMBP Tag Mouse Monoclonal Antibody (HRP Conjugated) The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Our anti-MBP monoclonal antibody provides a simple solution to detect the expression of protein ... no rear speakers with subWebDYKDDDDK Tag Monoclonal Antibody ... -Mouse Monoclonal Antibody Acquista DYKDDDDK Tag Monoclonal Antibody (FG4R), Biotin, Invitrogen™ su. Italiano … no rear lights arshttp://www.abpbiosciences.com/product/flag-tag/ how to remove green mark in excel cellWebJun 15, 2012 · The FLAG sequence (DYKDDDDK) is an artificial sequence widely used to detect, quantify, and purify proteins expressed as FLAG-fusion proteins. Several highly specific monoclonal antibodies for FLAG are commercially available; however, they are not always sensitive enough to detect proteins expressed … how to remove green highlight in onenote