웹2024년 1월 25일 · The cells (1 × 10 6) were lysed in 100 μl of assay buffer and then centrifuged under ice-cold conditions at 15,000 rpm for 10 min to pellet the insoluble materials. The supernatant was then collected, and 10–50 μl of this supernatant was added to a 96-well plate; the final volume was topped up to 50 μl/well with assay buffer. 웹Handling Advice. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
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웹16시간 전 · Glass slides with consecutive brain tissue sections were desiccated at room temperature for 20 min before the application of the derivatization and matrix solutions. Solutions of four coupling reagents (HATU (2 mM), HBTU (2 mM), EDCl (2 mM), and TOTU (2 mM), each of which contained the AMPP (2.5 mM) derivatization reagent) and TEA (1.5 … 웹1997년 5월 1일 · View raw image; Final state of run 1: homologous temperature at the ice base (in °C). The spacing between the isolines is 3°C. Open diamond symbols indicate positions where the basal ice is at the pressure melting point, yet with no temperate layer above; full diamonds (full circles, see → in the plot) indicate positions where there is a … early childhood research topics
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웹2024년 4월 13일 · Abrasion occurs when clasts entrained in basal ice are pressed against the bed of the glacier and mobilized by the sliding ice (Hallet, 1979 5. Hallet, B., ... we create the ice ring with the freezer temperature set to −6 °C. Ice made from de-ionized water is crushed with an industrial ice shaver (snow-cone maker) ... 웹2024년 3월 23일 · Figure 7 shows the plot of wet points on the model basal temperature map using a geothermal heat flux of 55 mW m −2 (a) and 60 mW m −2 (b) . The undetermined ice–bedrock interface mainly exists in the high basal temperature area, so only a few wet points are scattered in the area above −5 °C. 웹The basal {0001}, prismatic {101̅0}, pyramidal {202̅1}, and secondary prism {112̅0} facets of ice-Ih were drawn through liquid water with a momentum flux between the solid and liquid phases. css 隣り合う